Composite

Part:BBa_K1547000:Design

Designed by: victor yuan   Group: iGEM14_Concordia   (2014-10-08)


PSAD Promoter and Terminator, with GFP reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1570
    Illegal AgeI site found at 916
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1679


Design Notes

PCR SOEing and Gibson assembly was used.


Source

Constructed in vivo from individual parts.

References